I don't think, online server of autobuster is available. It is freely available, you can install it and run locally. https://www.globalphasing.com/buster/manual/autobuster/manual/index.html
Atul On 1/13/15, rohit kumar <[email protected]> wrote: > Hi, > Is Autobuster a online server? > On 13-Jan-2015 2:27 pm, "Atul Kumar" <[email protected]> wrote: > >> Dear Rohit, >> >> You can follow the way Tim suggested. At low resolution and for >> disordered >> regions, autobuster works well for refinement. >> >> Atul >> >> On Tue, Jan 13, 2015 at 7:47 AM, Tim Gruene <[email protected]> >> wrote: >> >>> Dear Rohit Kumar Singh, >>> >>> you can remove a couple of residues before and after the outlier and >>> rebuild it. You can also switch on Ramachandran restraints in Coot and >>> run real-space refinement. If this does not help, I would delete a >>> single residue within the region in question, run real-space refinement >>> for both segments and reconnect. >>> >>> Regards, >>> Tim >>> >>> On 01/13/2015 08:35 AM, rohit kumar wrote: >>> > Hello all, >>> > >>> > if outlier is in between 5 to 6 %, how someone can fix it. >>> > As the resolution is low (3.0-3.5 A). >>> > >>> > On Mon, Jan 12, 2015 at 7:53 PM, Robbie P. Joosten <[email protected]> >>> wrote: >>> > >>> >> Hi Dialing, >>> >> >>> >> 86% favoured can actually be quite okay for an initial model. As long >>> as >>> >> it doesn't have 14% in the disallowed region, the vast majority of >>> residues >>> >> will be correct, or close enough to the correct answer that they can >>> >> be >>> >> fixed easily. Of course, such a model will need tweaking, but this is >>> more >>> >> or less the point of calling it an 'initial' model. >>> >> >>> >> Cheers, >>> >> Robbie >>> >> >>> >> >>> >> On 01/12/2015 01:18 PM, Dialing Pretty wrote: >>> >> >>> >>> Dear All， >>> >>> >>> >>> If an initial PDB has only 86% residues in the Ramachandran favored >>> >>> region, it >>> >>> would mean there is a significant error (for example significant >>> length of >>> >>> protein fragment in the total protein assigned to the wrong electron >>> >>> density map >>> >>> position) , right? >>> >>> >>> >>> Dialing >>> >>> >>> >>> >>> > >>> > >>> >>> -- >>> Dr Tim Gruene >>> Institut fuer anorganische Chemie >>> Tammannstr. 4 >>> D-37077 Goettingen >>> >>> GPG Key ID = A46BEE1A >>> >>> >> >
