Dear William,
at the ESRF we have a Helical characterisation workflow that will calculate a strategy based on the observed diffraction patterns and the distance between 2 points selected on a crystal, best wishes, Matt.



On 2015-04-24 12:00, William Chao wrote:
Dear all,

Does anyone have experience in maximising anomalous signal during a
helical/line scan? Most (all?) automated data collection strategies in
synchrotrons seem to work on a single point of a crystal but don't
give strategies for line scan. Would it be advisable to increase the
recommended transmission as radiation is spread along the crystal? If
the strategy suggests us to use 10% transmission (100% = 2x10^11 ph/s) for a 360-degree collection on a single point, to what amount should I
increase say along a 200 micron scan with a 20 micron beam (crystal
dimension 200x100x20)?

Thank you very much in advance for the suggestions!

William
---

 The Francis Crick Institute Limited is a registered charity in
England and Wales no. 1140062 and a company registered in England and
Wales no. 06885462, with its registered office at 215 Euston Road,
London NW1 2BE.

--
Matthew Bowler
Synchrotron Diffraction Group
European Molecular Biology Laboratory
71, avenue des Martyrs
CS 90181
F-38042 GRENOBLE Cedex 9
FRANCE
France
===================================================
Tel: +33 (0) 4.76.20.76.37
Fax: +33 (0) 4.76.88.29.04

http://www.embl.fr/
===================================================

Reply via email to