Hi Matthew, William seems to want to increase the x-ray intensity gradually during the experiment by augmenting the transmission gradually. It that possible? If so, would it be advisable?
Mark J van Raaij Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/~mjvanraaij On 24 Apr 2015, at 12:45, Matthew BOWLER wrote: > Dear William, > at the ESRF we have a Helical characterisation workflow that will calculate a > strategy based on the observed diffraction patterns and the distance between > 2 points selected on a crystal, best wishes, Matt. > > > > On 2015-04-24 12:00, William Chao wrote: >> Dear all, >> >> Does anyone have experience in maximising anomalous signal during a >> helical/line scan? Most (all?) automated data collection strategies in >> synchrotrons seem to work on a single point of a crystal but don't >> give strategies for line scan. Would it be advisable to increase the >> recommended transmission as radiation is spread along the crystal? If >> the strategy suggests us to use 10% transmission (100% = 2x10^11 ph/s) >> for a 360-degree collection on a single point, to what amount should I >> increase say along a 200 micron scan with a 20 micron beam (crystal >> dimension 200x100x20)? >> >> Thank you very much in advance for the suggestions! >> >> William >> --- >> >> The Francis Crick Institute Limited is a registered charity in >> England and Wales no. 1140062 and a company registered in England and >> Wales no. 06885462, with its registered office at 215 Euston Road, >> London NW1 2BE. > > -- > Matthew Bowler > Synchrotron Diffraction Group > European Molecular Biology Laboratory > 71, avenue des Martyrs > CS 90181 > F-38042 GRENOBLE Cedex 9 > FRANCE > France > =================================================== > Tel: +33 (0) 4.76.20.76.37 > Fax: +33 (0) 4.76.88.29.04 > > http://www.embl.fr/ > ===================================================
