There's some discussion of this in here:
http://journals.iucr.org/d/issues/2013/07/00/ba5199/index.html
section 6.5.
But basically you figure out how much dose a volume can take, and then
change collection parameters so that each volume gets that full dose.
The "strategy" is just one way to quantify the dose; I personally like
to convert things back to "unattenuated seconds", i.e. how many seconds
will the crystal "survive" with the unattenuated beam (in your case,
anomalous signal rather than crystal).
phx
On 24/04/2015 11:00, William Chao wrote:
Dear all,
Does anyone have experience in maximising anomalous signal during a
helical/line scan? Most (all?) automated data collection strategies in
synchrotrons seem to work on a single point of a crystal but don't
give strategies for line scan. Would it be advisable to increase the
recommended transmission as radiation is spread along the crystal? If
the strategy suggests us to use 10% transmission (100% = 2x10^11 ph/s)
for a 360-degree collection on a single point, to what amount should I
increase say along a 200 micron scan with a 20 micron beam (crystal
dimension 200x100x20)?
Thank you very much in advance for the suggestions!
William
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