Dear Vikram

Your file aat000_03_05.200_v1_3 <http://rosettaserver.bakerlab.org/downloads/fragments/45590/aat000_03_05.200_v1_3> contains fragments of 3 residues length, the other one contains 9-residue fragments. Download these to your own computer and then, in the CCP4i interface to AMPLE, use the Browse buttons in the Fragment Files section to locate them as 3mers and 9mers respectively. Once you've told AMPLE where your Rosetta installation is, and filled in the other Input Files as normal, you're good to go.

Best wishes

Daniel

On 21/10/16 10:50, Vikram Dalal wrote:
Hi eveyone,

I want to run the ample in ccp4i. I have generated the files for my sequence using robetta fragment library server. But, I do not know how to use these fragments in in ccp4i (3 mers and 9 mers). I got these results after submission of my sequences in robetta.

Your results are available at:

Result Details
http://rosettaserver.bakerlab.org/fragmentqueue.jsp?id=45590 <http://rosettaserver.bakerlab.org/fragmentqueue.jsp?id=45590>

Download Fragment Files
http://rosettaserver.bakerlab.org/downloads/fragments/45590/aat000_03_05.200_v1_3 <http://rosettaserver.bakerlab.org/downloads/fragments/45590/aat000_03_05.200_v1_3> http://rosettaserver.bakerlab.org/downloads/fragments/45590/aat000_09_05.200_v1_3 <http://rosettaserver.bakerlab.org/downloads/fragments/45590/aat000_09_05.200_v1_3>
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Thanks & Regards,


VIKRAM DALAL
Research Scholar
Macromolecular Crystallographic Unit
Department of Biotechnology
Indian Institute of Technology
Roorkee (INDIA)







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Dr Daniel John Rigden                 Tel:(+44) 151 795 4467
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