Hi Praveen, 20 years ago I seemed to spend more time siliconising cover slips than setting up the Magic 50. We had special jigs to hold a couple of dozen slips for washing, drying and finally immersion in silane solution. This method was very, very tedious. An alternative method was to pull a vacuum over the slips scattered on a glass Petri dish in a desiccator containing around 50mls of silane solution in a beaker. The dish sat on top of the beaker and the vacuum from a water tap pump would make the silane solvent boil. This was marginally quicker and used less silane solution. Either way, each slip was then individually polished using lens tissue and the dust blown away using compressed air before storage. My own “quick and dirty” method was to fill a tall plastic measuring cylinder with solution and drop the slips in serially and let them tumble through the solution as they sank. The cylinder should be tall and have a diameter comparable to that of the cover slip. I’d do around 300 at a time like this. All methods require scattering the slips onto aluminium foil and giving them a quick bake at around 100DegC to get rid of excess silane solution and HCl immediately after treatment. I gave up polishing them and just used to wash the slips in HPLC grade ethanol before drying in the oven and storing (hiding) them. Whichever way you choose, you do need to do the siliconising in a fume cupboard.
Best wishes, David From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Praveen Kumar Tripathi Sent: 27 April 2017 10:26 To: [email protected] Subject: [ccp4bb] Sliconizing of cover-slips Dear all, sorry for off topic question. May i know if anybody uses homemade silinization of coverslips for protein crystallization purposes? I have purchased Sigmacote SL2-100 ml for silanizing coverslips for hanging drop protein crystallization setup. Please share your methods to siliconize coverslip using Sigmacote SL2-100 ml if anybody uses. Thanks in advance regards Praveen -- Praveen Kumar Tripathi PhD Research Scholar Kusuma School of Biological Sciences Indian Institute of Technology Delhi-110016 +91-9873625228 ________________________________ AstraZeneca UK Limited is a company incorporated in England and Wales with registered number:03674842 and its registered office at 1 Francis Crick Avenue, Cambridge Biomedical Campus, Cambridge, CB2 0AA. This e-mail and its attachments are intended for the above named recipient only and may contain confidential and privileged information. If they have come to you in error, you must not copy or show them to anyone; instead, please reply to this e-mail, highlighting the error to the sender and then immediately delete the message. For information about how AstraZeneca UK Limited and its affiliates may process information, personal data and monitor communications, please see our privacy notice at www.astrazeneca.com<https://www.astrazeneca.com>
