To me this begs the question: why do this at all? A cost issue? The Hampton coverslips work pretty well even with detergents that drastically lower surface tension like C8E4. Those of a UK competitor that i shall not name are not as good, in our humble experience.
Bert ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of Tristan Croll <[email protected]> Sent: 28 April 2017 12:36 To: [email protected] Subject: Re: [ccp4bb] Sliconizing of cover-slips Ahh, this brings back memories of a former life, preparing hydrophobic coverslips for my surface chemistry experiments. I used chlorotrimethylsilane, and what I remember best is that the secret to a good coating is making sure your coverslips are utterly clean and dry. I used to clean them by boiling in base piranha (concentrated ammonia and hydrogen peroxide - need I say this stuff should be treated with extreme respect?) before rinsing thoroughly in milli-Q water and baking under vacuum. Then essentially as David said: dump them in the silane solution (again, it helps to make sure to keep your solvent dry), then fish them out one-by-one, rinse and dump them into a beaker of fresh solvent (acetone, I think I used). Then once they're all done, take them out of that, dry in a nitrogen stream and store. Painful & fiddly, but you can easily do a hundred or so in an afternoon. Tristan Croll Research Fellow Cambridge Institute for Medical Research University of Cambridge CB2 0XY On 27 Apr 2017, at 10:26, Praveen Kumar Tripathi <[email protected]<mailto:[email protected]>> wrote: Dear all, sorry for off topic question. May i know if anybody uses homemade silinization of coverslips for protein crystallization purposes? I have purchased Sigmacote SL2-100 ml for silanizing coverslips for hanging drop protein crystallization setup. Please share your methods to siliconize coverslip using Sigmacote SL2-100 ml if anybody uses. Thanks in advance regards Praveen -- Praveen Kumar Tripathi PhD Research Scholar Kusuma School of Biological Sciences Indian Institute of Technology Delhi-110016 +91-9873625228
