Dear Praveen, I always siliconize my cover slips before using them for crystallization. Recipe is very simple. But make sure that you always use mask, gloves and perform your experiment under a fume hood.
1. 95% toluene + 5% dichloromethylsilane will give you a very good siliconization solution. 2. Purchase thick cover slips ( because thin one is very fragile and can break during the experiment) and wash them with water to remove the dust. Then wash them with acetone and keep them hot air oven for drying. 3. Then take a large glass petri dish and pore the siliconization solution. Then put all cover slips into it and keep them for 30sec-1min.( do this step inside the hood ). Make sure that all faces of cover slip can come to the contact of siliconization solution. 4. Then take each cover slip one by one with tweezer and wash them with toluene ( in other petri dish) then put them on water ( They will float if they are siliconized and it is a proof of hydrophobic coat on them). 5. Collect them and keep them for drying in hot air oven for few hours. If you find any problem, I will happy to discus with you. Best of luck! Regards Jaydeep Paul. Project fellow at NISER,Bhubaneswar,India Dr. Rudresh Acharya's lab ᐧ On Thu, Apr 27, 2017 at 2:56 PM, Praveen Kumar Tripathi < [email protected]> wrote: > Dear all, > > sorry for off topic question. > > May i know if anybody uses homemade silinization of coverslips for protein > crystallization purposes? > > I have purchased Sigmacote SL2-100 ml for silanizing coverslips for > hanging drop protein crystallization setup. > > Please share your methods to siliconize coverslip using Sigmacote SL2-100 > ml if anybody uses. > > Thanks in advance > > regards > Praveen > > > > -- > *Praveen Kumar Tripathi* > PhD Research Scholar > Kusuma School of Biological Sciences > Indian Institute of Technology Delhi-110016 > +91-9873625228 >
