Dear Nishant, Rosetta is a good suggestion. You can also use an ensemble of several related (superposed) structures as your search model. This will improve your chances of success.
All best. Andreas On Tue, Aug 29, 2017 at 12:50 PM, Nishant Varshney <[email protected]> wrote: > Dear Crystallographers, > > I am working to solve an human protein structure which has a domain > sequence identity of 24% with domain of another protein. As Phaser as well > as Molrep failed to give any definite solution (TFZ=3.7 from MR), I want to > ask, if solution structure of another protein having domain sequence > similarity of 25% or a homology model can be used as a template for MR? > Many thanks and Regards > Nishant > > -- > Dr. Nishant Kumar Varshney, > Research Associate, > C/O Dr. Sameena Khan, > Drug Discovery Research Center, > Translational Health Science and Technology Institute (THSTI) > NCR Biotech Science Cluster, > 3rd Milestone, Faridabad – Gurgaon Expressway, > Faridabad – 121001 (HARYANA), India > Ph: +91- 0129-2876477 <+91%20129%20287%206477> > Mob: 8390564690 >
