Hi Herman, I haven't done His-6 versus His-10 for the same protein, but have done that for different ones with success. However, if in His-6 containing protein structure the packing or folding is such that you don't see His-6 then it shouldn't matter it is 6 or 10. Just an opinion.
Regards, Vaheh Oganesyan www.medimmune.com From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of [email protected] Sent: Tuesday, September 19, 2017 6:11 AM To: [email protected] Subject: [ccp4bb] His-6 versus His-10 tag Dear BB, We are planning the production of a protein for crystallization. From literature, we know that the construct with a 6-histidine tag crystallizes. However, for other biophysical measurements, we would prefer to have a 10-histidine tag. Does anyone has experience with His-6 versus His-10 tags in terms of crystallization success? Thanks for your help! Herman To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation.
