Hi Herman, I worked on a kinase, where moved from 6-His in the literature to 8-His, and it didn't impact crystallization or diffraction (which around 3Angs).
Good Luck Partha On Tue, Sep 19, 2017 at 9:43 AM Oganesyan, Vaheh <[email protected]> wrote: > Hi Herman, > > > > I haven’t done His-6 versus His-10 for the same protein, but have done > that for different ones with success. However, if in His-6 containing > protein structure the packing or folding is such that you don’t see His-6 > then it shouldn’t matter it is 6 or 10. Just an opinion. > > > > *Regards,* > > > > *Vaheh Oganesyan* > > *www.medimmune.com <http://www.medimmune.com>* > > > > *From:* CCP4 bulletin board [mailto:[email protected]] *On Behalf Of * > [email protected] > *Sent:* Tuesday, September 19, 2017 6:11 AM > *To:* [email protected] > *Subject:* [ccp4bb] His-6 versus His-10 tag > > > > Dear BB, > > > > We are planning the production of a protein for crystallization. From > literature, we know that the construct with a 6-histidine tag crystallizes. > However, for other biophysical measurements, we would prefer to have a > 10-histidine tag. > > > > Does anyone has experience with His-6 versus His-10 tags in terms of > crystallization success? > > > > Thanks for your help! > > Herman > > > > > To the extent this electronic communication or any of its attachments > contain information that is not in the public domain, such information is > considered by MedImmune to be confidential and proprietary. This > communication is expected to be read and/or used only by the individual(s) > for whom it is intended. If you have received this electronic communication > in error, please reply to the sender advising of the error in transmission > and delete the original message and any accompanying documents from your > system immediately, without copying, reviewing or otherwise using them for > any purpose. Thank you for your cooperation. >
