Option 1: make two constructs and compare :) Option 2: put the his10 on the n-term of the protein with a cleavage site
In my experience it matters. I had cases where his4 crystallized and his6 did not. I also had the opposite experience. Artem On Sep 19, 2017 6:11 AM, <[email protected]> wrote: Dear BB, We are planning the production of a protein for crystallization. From literature, we know that the construct with a 6-histidine tag crystallizes. However, for other biophysical measurements, we would prefer to have a 10-histidine tag. Does anyone has experience with His-6 versus His-10 tags in terms of crystallization success? Thanks for your help! Herman
