Hello All, I am currently working on the structure of a DNA protein complex. The data has been processed in space group P1 (53.042 59.527 78.526 105.24 98.03 106.99 P 1, Rpim 11.7%). At this stage I have almost 85% model is complete but my R/Rfree is stuck as 26%/34%.
I have some concerns and questions: * Xtriage says there are a large number of outliers; however no pseudotranslational symmetry is detected by the program. What are the other reasons for outliers? * I am trying phenix.refine for refinement with the default settings. Is there any special setting that can help me? I would like to have some suggestions about my problem. Thanks in advance Radhika
