Dear Radhika, What reason does Xtriage give for declaring the reflections to be outliers? Too weak, too strong, other reasons? As was mentioned before, what is the resolution of your data? In cases like this, it is always good to have a look at the diffraction images to see if there is some problem there like streaks, ice rings etc.
Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Radhika Singh Gesendet: Dienstag, 14. November 2017 00:45 An: CCP4BB@JISCMAIL.AC.UK Betreff: [EXTERNAL] [ccp4bb] High R/Rfree Hello All, I am currently working on the structure of a DNA protein complex. The data has been processed in space group P1 (53.042 59.527 78.526 105.24 98.03 106.99 P 1, Rpim 11.7%). At this stage I have almost 85% model is complete but my R/Rfree is stuck as 26%/34%. I have some concerns and questions: * Xtriage says there are a large number of outliers; however no pseudotranslational symmetry is detected by the program. What are the other reasons for outliers? * I am trying phenix.refine for refinement with the default settings. Is there any special setting that can help me? I would like to have some suggestions about my problem. Thanks in advance Radhika
