Remember DNA can generate some very strong reflections along the stacking axis.
But 2outliers" can mean anything - as Herman says look at the images. Aimless shows you a plot of where they are - and gives a list.. - they may be associated with ice rings? or just diffraction problems. On 14 November 2017 at 08:24, <[email protected]> wrote: > Dear Radhika, > > > > What reason does Xtriage give for declaring the reflections to be > outliers? Too weak, too strong, other reasons? As was mentioned before, > what is the resolution of your data? In cases like this, it is always good > to have a look at the diffraction images to see if there is some problem > there like streaks, ice rings etc. > > > > Best, > > Herman > > > > *Von:* CCP4 bulletin board [mailto:[email protected]] *Im Auftrag von > *Radhika Singh > *Gesendet:* Dienstag, 14. November 2017 00:45 > *An:* [email protected] > *Betreff:* [EXTERNAL] [ccp4bb] High R/Rfree > > > > Hello All, > > > > I am currently working on the structure of a DNA protein complex. The > data has been processed in space group P1 (53.042 59.527 78.526 105.24 > 98.03 106.99 P 1, Rpim 11.7%). At this stage I have almost 85% model is > complete but my R/Rfree is stuck as 26%/34%. > > > > I have some concerns and questions: > > * Xtriage says there are a large number of outliers; however no > pseudotranslational symmetry is detected by the program. What are the > other reasons for outliers? > > > > * I am trying phenix.refine for refinement with the default settings. Is > there any special setting that can help me? > > > > I would like to have some suggestions about my problem. > > > > Thanks in advance > > > > Radhika > > >
