I would recommend to look at the GE Superose6 increase 3.2/300 columns if you aim at high resolution with small sample volumes (you will be very limited in flow rate though).
For a much faster analysis with sufficient resolution, try the 5/150GL columns. Attached to an HPLC you can have runtimes of about 10 min per sample with decent resolution. ________________________________ Von: CCP4 bulletin board <[email protected]> im Auftrag von Artem Evdokimov <[email protected]> Gesendet: Samstag, 28. April 2018 22:16 An: [email protected] Betreff: Re: [ccp4bb] size exclusion columns Xk10/300 packed with superdex of the appropriate pore size is by far the most economical and robust option :) pack your own and save. Artem On Thu, Apr 26, 2018, 12:06 Markus Heckmann <[email protected]<mailto:[email protected]>> wrote: Dear all, We are looking for a size exclusion chromatography column (silica-based) for protein purification prior to a MALS-detector. We looked for (www.waters.com<http://www.waters.com> BEH-450), Sepax (Unix-C 300) and Phenomex (BioZen SEC-3). Any 'column' tips or recommendations when dealing with large proteins (MDa)? Many thanks Markus
