Hi Markus, just be aware that silica-based SEC columns are very sensitive to alkaline pH conditions, so you should not use them at pH higher that 7.5 We found that to be a limitation and thus chose polymer-based columns. Hth, ciao, Sebastiano
> On 26 Apr 2018, at 18:03, Markus Heckmann <[email protected]> wrote: > > Dear all, > > We are looking for a size exclusion chromatography column > (silica-based) for protein purification prior to a MALS-detector. We > looked for (www.waters.com BEH-450), Sepax (Unix-C 300) and Phenomex > (BioZen SEC-3). Any 'column' tips or recommendations when dealing > with large proteins (MDa)? > > Many thanks > Markus -- Sebastiano Pasqualato, PhD Crystallography Unit Department of Experimental Oncology European Institute of Oncology IFOM-IEO Campus via Adamello, 16 20139 - Milano Italy tel +39 02 9437 5167 fax +39 02 9437 5990
