I like the Xbridge columns from Waters, but for SEC-MALS you can also use Superose 6 Increase (GE), which will take longer to equilibrate and will go a bit noisier than a silica-based column, but might be advantageous in case a number of your proteins decide to interact with the silica resin.
Concerning the pH, you can use an Xbridge (modified silica) up to pH 8.0 or even pH 8.5 but keep in mind you are reducing the life of the column by doing that. Don't forget to ask the people who sold you the MALS detector, the serious companies always have someone to give good advice. On Thu, Apr 26, 2018 at 6:03 PM, Markus Heckmann <[email protected]> wrote: > Dear all, > > We are looking for a size exclusion chromatography column > (silica-based) for protein purification prior to a MALS-detector. We > looked for (www.waters.com BEH-450), Sepax (Unix-C 300) and Phenomex > (BioZen SEC-3). Any 'column' tips or recommendations when dealing > with large proteins (MDa)? > > Many thanks > Markus >
