Artem (and Beatriz),
Me bad, could have thought about that! I think you are right. There were initially bubbles in each drop (7 in one case, 4 in the other). At some point the bubbles exploded (it was an instantaneous process, not just shrinking). Kind regards, Sergei Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of Pharmaceutical Sciences, KU Leuven O&N2, Campus Gasthuisberg, Herestraat 49 bus 822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 32 Lab pages: http://pharm.kuleuven.be/Biocrystallography<http://pharm.kuleuven.be/anafar> ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of Artem Evdokimov <[email protected]> Sent: Thursday, March 28, 2019 1:07 To: [email protected] Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat! Looks like multiple adjacent bubbles that were initially touching but eventually shrunk down to the central cores - the connectors are protein filaments (skin on the bubbles) left over from when bubbles had contact points. Artem On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville) <[email protected]> wrote: Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition is found in both Index and JCSG screens as well as Classics II. Bevan Marshall Staff Scientist | Collaborative Crystallisation Centre Manufacturing CSIRO E [email protected]<mailto:[email protected]>T +61 3 9662 7492 343-351 Royal Parade, Parkville, VIC 3052 www.csiro.au<http://www.csiro.au/> | https://c3.csiro.au<https://c3.csiro.au/> CSIRO acknowledges the Traditional Owners of the lands that we live and work on across Australia and pays its respect to Elders past and present. PLEASE NOTE The information contained in this email may be confidential or privileged. Any unauthorised use or disclosure is prohibited. If you have received this email in error, please delete it immediately and notify the sender by return email. Thank you. To the extent permitted by law, CSIRO does not represent, warrant and/or guarantee that the integrity of this communication has been maintained or that the communication is free of errors, virus, interception or interference. Please consider the environment before printing this email. From: CCP4 bulletin board [mailto:[email protected]<mailto:[email protected]>] On Behalf Of LEGRAND Pierre Sent: Thursday, 28 March 2019 9:13 AM To: [email protected]<mailto:[email protected]> Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Dear Beatriz, Nice drops :-)) Could it be that there is a reaction going on in these drops ? The conditions are quite "exotic" with possibilities of coordination or oxydoreduction (Co2+/Co3+) or polymerization... Do you have reductants with the protein buffer ? Is the protein an enzyme or a metalloprotein ? Just some ideas. Best wishes, Pierre ________________________________ De : CCP4 bulletin board [[email protected]<mailto:[email protected]>] de la part de Beatriz Gomes Guimaraes [[email protected]<mailto:[email protected]>] Envoyé : mercredi 27 mars 2019 19:44 À : [email protected]<mailto:[email protected]> Objet : [ccp4bb] Interesting pattern on a crystallization drop Dear all, I would like to share with you a surprising pattern I found when examining some crystallization plates (attached figures). It is less obvious looking the photos, but apparently the "lines" are formed by precipitated protein and there are some "bubbles" with small drops inside. I wish they were microcrystals but I do not think this is the case. I was suprised by the symmetry ! And it is not completely random because for the same condition the difference between the two drops are : protein alone ("hexagon") and protein + ligand ("rhombus") crystallization condition is: 0.01 M Cobalt(II) chloride hexahydrate 0.1 M Tris pH 8.5 20% w/v Polyvinylpyrrolidone K 15 Have you seen anything similar before? Thank you for your comments! Beatriz -------------------------- Beatriz Guimarães Laboratory of Structural Biology and Protein Engineering Instituto Carlos Chagas - ICC / FIOCRUZ Paraná Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C CIC 81350-010 Curitiba - PR, Brasil Tel.:+55(41)3316-3225/2104-3438 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
