May be, I do co-relate your crystal pic with Manu Prakash at Stanford on his
work on Dancing Droplets, briefing the surface tension and evaporation ^ the
rule of two component fluids. # Since your precipitant contain PVP a shape
controlling agent
#https://med.stanford.edu/news/all-news/2015/03/researchers-solve-mystery-of-the-dancing-droplets.html
Best wishes
S.M.Jaimohan PhD
On Thursday, 28 March, 2019, 1:54:23 pm IST, Sergei Strelkov
<[email protected]> wrote:
#yiv3861306982 #yiv3861306982 --P{margin-top:0;margin-bottom:0;}#yiv3861306982
Artem (and Beatriz),
Me bad, could have thought about that! I think you are right.
There were initially bubbles in each drop (7 in one case, 4 in the other).
At some point the bubbles exploded (it was an instantaneous process, not just
shrinking).
Kind regards,
Sergei
Prof. Sergei V. Strelkov Laboratory for Biocrystallography Department of
Pharmaceutical Sciences, KU Leuven O&N2, Campus Gasthuisberg, Herestraat 49 bus
822, 3000 Leuven, Belgium Phone: +32 16 33 08 45, mobile: +32 486 29 41 32 Lab
pages: http://pharm.kuleuven.be/BiocrystallographyFrom: CCP4 bulletin board
<[email protected]> on behalf of Artem Evdokimov <[email protected]>
Sent: Thursday, March 28, 2019 1:07
To: [email protected]
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop Neat!
Looks like multiple adjacent bubbles that were initially touching but
eventually shrunk down to the central cores - the connectors are protein
filaments (skin on the bubbles) left over from when bubbles had contact points.
Artem
On Wed, Mar 27, 2019, 19:39 Marshall, Bevan (Manufacturing, Parkville)
<[email protected]> wrote:
Looked up the condition on C6 (https://c6.csiro.au/C6.asp) and that condition
is found in both Index and JCSG screens as well as Classics II.
Bevan Marshall
Staff Scientist | Collaborative Crystallisation Centre
Manufacturing
CSIRO
E [email protected] +61 3 9662 7492
343-351 Royal Parade, Parkville, VIC 3052
www.csiro.au|https://c3.csiro.au
CSIRO acknowledges the Traditional Owners of the lands that we live and work on
across Australia and pays its respect to Elders past and present.
PLEASE NOTE
The information contained in this email may be confidential or privileged. Any
unauthorised use or disclosure is prohibited. If you have received this email
in error, please delete it immediately and notify the sender by return email.
Thank you. To the extent permitted by law, CSIRO does not represent, warrant
and/or guarantee that the integrity of this communication has been maintained
or that the communication is free of errors, virus, interception or
interference.
Please consider the environment before printing this email.
From: CCP4 bulletin board [mailto:[email protected]]On Behalf Of LEGRAND
Pierre
Sent: Thursday, 28 March 2019 9:13 AM
To: [email protected]
Subject: Re: [ccp4bb] Interesting pattern on a crystallization drop
Dear Beatriz,
Nice drops :-))
Could it be that there is a reaction going on in these drops ?
The conditions are quite "exotic" with possibilities of coordination or
oxydoreduction (Co2+/Co3+) or polymerization...
Do you have reductants with the protein buffer ?
Is the protein an enzyme or a metalloprotein ?
Just some ideas.
Best wishes,
Pierre
De : CCP4 bulletin board [[email protected]] de la part de Beatriz Gomes
Guimaraes [[email protected]]
Envoyé : mercredi 27 mars 2019 19:44
À : [email protected]
Objet : [ccp4bb] Interesting pattern on a crystallization drop
Dear all,
I would like to share with you a surprising pattern I found when examining some
crystallization plates (attached figures).
It is less obvious looking the photos, but apparently the "lines" are formed by
precipitated protein and there are some "bubbles" with small drops inside.I
wish they were microcrystals but I do not think this is the case.
I was suprised by the symmetry !
And it is not completely random because for the same condition the difference
between the two drops are : protein alone ("hexagon") and protein + ligand
("rhombus")
crystallization condition is:
0.01 M Cobalt(II) chloride hexahydrate
0.1 M Tris pH 8.5
20% w/v Polyvinylpyrrolidone K 15
Have you seen anything similar before?
Thank you for your comments!
Beatriz
--------------------------
Beatriz Guimarães
Laboratory of Structural Biology and Protein Engineering
Instituto Carlos Chagas - ICC / FIOCRUZ Paraná
Rua Prof. Algacyr Munhoz Mader, 3775 Bloco C
CIC 81350-010
Curitiba - PR, Brasil
Tel.:+55(41)3316-3225/2104-3438
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
