How about BioGel-A50m with an exclusion limit of 50 million Da? I've used A15m for complexes as large as 500 kDa and it seemed to work well.
__________________ Roger Rowlett On Wed, Apr 17, 2019, 11:11 AM Mohinder Pal <[email protected]> wrote: > Dear all, > > I would like to gel filter a multi protein complex (1.4MDa) as the final > purification step. I have tried tandem Superose 6 columns and this complex > elutes close to void volume as it is a very elongated molecule. > > > I was wondering if someone could suggest different analytical columns for > better resolution as I plan to add more components to this existing > complex. > > Best wishes, > > Mohinder Pal > > ---------------------------------------------- > "Whatever you’re meant to do, do it now. The conditions are > always impossible.” > Doris Lessing > ---------------------------------------------- > > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
