Hi Mohinder,
if you are up to trying something new the monolithic columns by Biaseparation (analytical or prep) are well suited for big molecules / particles and worked very well for me. The fittings work with the GE chromatography instruments. https://www.biaseparations.com Good luck, Bärbel -- Bärbel Blaum, PhD Inthera Bioscience AG Einsiedlerstrasse 34 CH-8820 Waedenswil Switzerland E-Mail: [email protected] Phone: +41 43 477 94 72-- Von: CCP4 bulletin board <[email protected]> im Auftrag von David Blum <[email protected]> Antworten an: David Blum <[email protected]> Datum: Mittwoch, 17. April 2019 um 21:48 An: <[email protected]> Betreff: Re: [ccp4bb] analytical gel filtration columns Mohinder, You could also try HPLC where you should get better results due to more theoretical plates. One option would from Phenomenex. 10E6A Phase Information · Molecular Weight Range: 60 K - 10,000 K https://www.phenomenex.com/Products/HPLCDetail/phenogel David L. Blum, Ph.D. Bioexpression and Fermentation Facility | Director Master of Biomanufacturing and Bioprocessing | Director 120 Green Street | Life Sciences Bldg rm A414A | Athens, GA 30602 706-542-1035 | [email protected] | bff.uga.edu | biomanufacturing.uga.edu On Wed, Apr 17, 2019 at 11:11 AM Mohinder Pal <[email protected]> wrote: Dear all, I would like to gel filter a multi protein complex (1.4MDa) as the final purification step. I have tried tandem Superose 6 columns and this complex elutes close to void volume as it is a very elongated molecule. I was wondering if someone could suggest different analytical columns for better resolution as I plan to add more components to this existing complex. Best wishes, Mohinder Pal ---------------------------------------------- "Whatever you’re meant to do, do it now. The conditions are always impossible.” Doris Lessing ---------------------------------------------- To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
