Hi Almuda, in addition to everything that had been said, I would have a look at all the images of your data set, to see if there is anything that may be causing misindexing in addition to the streaky spots. ADXV has the option to play a movie of a complete data set. Also, ORGX and ORGY must be accurate; as a crude estimate, the error should be less than half the distance between to diffraction spots. Also, where Clemens was hinting at, damaged detector pixels that are not removed from the image may jeopardize the indexing. Best, Herman
Von: Almudena Ponce Salvatierra <[email protected]> Gesendet: Samstag, 30. November 2019 11:00 An: Schreuder, Herman /DE <[email protected]> Cc: [email protected] Betreff: Re: [EXTERNAL] [ccp4bb] 8/ 2263 spots indexed XDS EXTERNAL : Real sender is [email protected]<mailto:[email protected]> Hi Herman, thank you for your answer. Yes, my data were collected at the synchrotron, however, the autoprocessing failed with some of the datasets. I have checked the image headers and the XDS.INP files, trying to find any mismatches concerning origin, wavelength, etc... but it seems that the information was recorded properly. I am trying different origins with one of the datasets, but it has not helped so far. I will write back once I find out what the problem was. Best wishes, Almudena El vie., 29 nov. 2019 a las 14:07, Schreuder, Herman /DE (<[email protected]<mailto:[email protected]>>) escribió: Dear Almu, were your data collected at a synchrotron? Many synchrotrons run automatic processing of the data being collected and it is worth looking if there is some processing directory between the frames you collected. If so, these data may already be quite decent, and in any case, the XDS.INP file may tell you how to set the optimal processing parameters for this particular beamline. You can also look at the refined parameters of your successful XDS run, especially the ORGX and ORGY. Try to rerun XDS, using the refined parameters of the successful run. Good luck! Herman Von: CCP4 bulletin board <[email protected]<mailto:[email protected]>> Im Auftrag von Almudena Ponce Salvatierra Gesendet: Freitag, 29. November 2019 12:48 An: [email protected]<mailto:[email protected]> Betreff: [EXTERNAL] [ccp4bb] 8/ 2263 spots indexed XDS EXTERNAL : Real sender is [email protected]<mailto:[email protected]> Dear all, I have some data sets that don't want to be processed :p In one of them, when I look at IDXREF.LP I see virtually none of the found spots were indexed and the reason is that they are "too far from the expected position". The spots are smeary and elongated, so not the prettiest. I have managed to process so far only one data set with decent statistics from another crystal harvested from the same drop, where the diffraction spots look better. I am trying to find in the xds wiki the keyword I should fine tune in order to make those spots indexable. Could you help me please? Thank you very much in advance. Best wishes, Almu ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=HK-CY_tL8CLLA93vdywyu3qI70R4H8oHzZyRHMQu1AQ&m=OmNNccFfd0ncUhz71B-ZuPSz9zldaXv5adAn5pWyo-0&s=vbh508VYVlXuCpniEp-8Z-n_5rMz1Ae4Tf0jMF2mSWw&e=> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
