5FRT We got scooped on this one a long time ago - but back in 2015 it was very gratifying to see that the academic group who published this structure have also obtained an ASU with two dimers and one monomer - same exact story as what we saw. The difference can be attributed to the movement of a short helix.
Nature is cruel, but interesting. Artem On Fri, Jan 31, 2020, 10:33 AM Kluenemann, Thomas < thomas.kluenem...@helmholtz-hzi.de> wrote: > Dear all, > > > > We recently solved a the structure of a small c-type cytochrome. We > observed, that of the eleven chains in the asymmetric unit ten form 3D > domain swapped dimers by exchanging an α-helix. The eleventh chain is > present as a monomer. Based on the anomalous iron signal and the chain > tracing we are sure that no chain was missed. > > I tried to find other examples in the PDB, were one crystal is made of > different homo- or heterooligomers. I only found proteins with partial > occupied peptide binding sites, which is not what I am looking for. Does > anyone know of a case were the presence of different homo- or > heterooligomers is required to form the crystal? > > > > Best regards, > > Thomas Klünemann > > > > > > ------------------------------ > > Helmholtz-Zentrum für Infektionsforschung GmbH | Inhoffenstraße 7 | 38124 > Braunschweig | www.helmholtz-hzi.de > > Vorsitzende des Aufsichtsrates: Frau MinDir'in Prof. Dr. Veronika von > Messling > Stellvertreter: MinDirig Rüdiger Eichel, Niedersächsisches Ministerium für > Wissenschaft und Kultur > Geschäftsführung: Prof. Dr. Dirk Heinz; Silke Tannapfel > Gesellschaft mit beschränkter Haftung (GmbH) > Sitz der Gesellschaft: Braunschweig > Handelsregister: Amtsgericht Braunschweig, HRB 477 > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
