Dear Laura, a few years ago - when I realised that mamalian cell expression media *without Met* costed much more than *with Met* because it is classified as custom media - I tried to persuade the rep that there was a market for it - to no avail.
I did then plan an experiment in which I would deplete the media of Met using this enzyme : https://en.wikipedia.org/wiki/Methionine_gamma-lyase and then supplement the Met-depleted media with Se-Met. At the time I discussed purchase of Met gamma-lyase from: Alpha Laboratories Web: www.alphalabs.co.uk<http://www.alphalabs.co.uk/> Before I could attempt the experiment - a Pt salt then came to my rescue and I was able to calculate initial phases by SAD with a diffraction experiment at a Pt-edge wavelength - so I cannot tell you if the idea of generating my own media would have worked. Just in case it helps! Ciao Pietro Pietro Roversi Lecturer (Teaching and Research) https://le.ac.uk/natural-sciences/ LISCB Wellcome Trust ISSF Fellow <https://bit.ly/2I4Wm5Z>https://le.ac.uk/liscb/research-groups/pietro-roversi Leicester Institute of Structural and Chemical Biology Department of Molecular and Cell Biology, University of Leicester Henry Wellcome Building Lancaster Road, Leicester, LE1 7HB England, United Kingdom Skype: roversipietro Mobile phone +44 (0) 7927952047 Tel. +44 (0)116 2297237 ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of LAURA DEL AMO MAESTRO <[email protected]> Sent: 10 March 2020 17:40 To: [email protected] <[email protected]> Subject: [ccp4bb] Expi 293 (Met-) expression medium for selenomethionine labeling Dear ccp4 community, I am in my last 3 months of my PhD, working at the Structural Biology Unit at IBMB-CSIC in Barcelona. As my experimental phasing trials using various heavy metals failed so far, we ordered two months ago Expi 293 (Met-) expression medium for selenomethionine labeling (see the reference: https://www.thermofisher.com/order/catalog/product/A4096701#/A4096701<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.thermofisher.com%2Forder%2Fcatalog%2Fproduct%2FA4096701%23%2FA4096701&data=02%7C01%7Cpr159%40LEICESTER.AC.UK%7C15b8a2ea1a0045a5078c08d7c51ba404%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C1%7C637194594816063263&sdata=kFB5h5yHX8d%2FfCqjmrxkhwzsx6coT4S9odKupAvhELU%3D&reserved=0>. But we got now the notice that it will not be delivered until end of April. I had been working so hard on this project, and this medium is essential to finished with the experimental part of my thesis. Thus, I was wondering if someone in the community has a bottle (1L) of this expression medium without methionine that I could borrow, and which I would return immediately once our medium arrives. Also in case you have recommendations or other sources of a defined Se-Met medium for Expi293 cells, please let me know. With kind regards from Barcelona, and hope someone can help, Laura --- Laura del Amo Maestro - Proteolysis Lab SBU-Structural Biology Unit, IBMB CSIC Barcelona, Spain, Europe ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.jiscmail.ac.uk%2Fcgi-bin%2Fwebadmin%3FSUBED1%3DCCP4BB%26A%3D1&data=02%7C01%7Cpr159%40LEICESTER.AC.UK%7C15b8a2ea1a0045a5078c08d7c51ba404%7Caebecd6a31d44b0195ce8274afe853d9%7C0%7C1%7C637194594816073262&sdata=ToNLBOeQiUTrLs%2BRRlg6v6hmJiDzVwbjiyZmz99gVHw%3D&reserved=0> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
