Hi Laura,

For what it’s worth, you’re not alone. I ordered two bottles back in January 
and have gotten availability dates ranging from March to May. However, it might 
be worth calling Thermo and checking with them on your specific order.

Last week I was told that they had a batch that was undergoing QC and I just 
got off the phone with someone who told me that my bottles were shipping today! 
Depending on where you are in the queue, you may have a shipment coming too.


Good luck!

Grant

- - - - - - - - - - - - -
Grant Weaver
PhD Candidate
Stern Lab
UMass Medical School


From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> On Behalf Of LAURA DEL AMO 
MAESTRO
Sent: Tuesday, March 10, 2020 1:41 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Expi 293 (Met-) expression medium for selenomethionine 
labeling

Dear ccp4 community,

I am in my last 3 months of my PhD, working at the Structural Biology Unit at 
IBMB-CSIC in Barcelona. As my experimental phasing trials using various heavy 
metals failed so far, we ordered two months ago Expi 293 (Met-) expression 
medium for selenomethionine labeling (see the reference: 
https://www.thermofisher.com/order/catalog/product/A4096701#/A4096701<https://nam01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.thermofisher.com%2Forder%2Fcatalog%2Fproduct%2FA4096701%23%2FA4096701&data=02%7C01%7Cgrant.weaver%40UMASSMED.EDU%7Cedea59bf9e894204d47f08d7c51ba084%7Cee9155fe2da34378a6c44405faf57b2e%7C0%7C1%7C637194594776359317&sdata=z2xjtrwahJpgu5xEtLlse%2FvlQ6h6O2781d%2BIPHercKo%3D&reserved=0>.
 But we got now the notice that it will not be delivered until end of April.

I had been working so hard on this project, and this medium is essential to 
finished with the experimental part of my thesis. Thus, I was wondering if 
someone in the community has a bottle (1L) of this expression medium without 
methionine that I could borrow, and which I would return immediately once our 
medium arrives.

Also in case you have recommendations or other sources of a defined Se-Met 
medium for Expi293 cells, please let me know. With kind regards from Barcelona, 
and hope someone can help,

Laura

---
Laura del Amo Maestro - Proteolysis Lab
SBU-Structural Biology Unit, IBMB CSIC
Barcelona, Spain, Europe

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