Re: [ccp4bb] Highest resolution of X-ray / neutron / electron crystallography, cryo EM

Tue, 09 Jun 2020 07:25:30 -0700

Hi Tobias,

For what is worth, we report 1.22 A for single particle cryo-EM ;-)) But very likely there is more in that dataset, we should know soon.

Best wishes,
Radu



On 9 Jun 2020 3:11 pm, Tobias Beck <[email protected]> wrote:
Dear all,

Thanks a lot! (I should have used the PDB query myself for neutrons, sry, my bad)

As there was a request to share the bioRxiv links, here they are:

along with the comment in Nature (which also has both links to the papers in the references section):

So:
X-ray at 0.48 A
Neutron at 0.93 A (hybrid with X-ray) or 1.05 A
Cryo EM 1.25 A
electron diffraction 0.6 A

Thanks to all of you!

Best, Tobias.


On Tue, Jun 9, 2020 at 3:46 PM Tobias Beck <[email protected]> wrote:
Dear all,

Thanks for the link to the latest BioRxiv papers! So for cryo EM it is 1.2 now. Any numbers for neutron?

Best, Tobias. 

Tobias Beck <[email protected]> schrieb am Di. 9. Juni 2020 um 15:35:
Dear all,

I was asked by a student what the highest resolution is, for each of the four methods listed above. Maybe someone has researched the current numbers previously and would like to share them? For X-ray, I found 0.48 A in the PDB. For EM method details, the PDB gives me 0.6 A, but it is actually for electron diffraction. I found a structure with 1.8 A for Cryo EM.

I am aware that resolution is only one parameter and that high resolution may not correspond to high data quality. However, maybe someone knows the record holders, either for biomacromolecules or small molecules or for both.

Thanks!

Best, Tobias.




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