Dear Tobias,
Thank you for identifying the 0.6A resolution structure solved by
electron diffraction as 6KJ3. This is a good, genuine de novo structure
determination using direct methods (SHELXD) on high-resolution data. The
R-values are not flattering (above 30%) but one can't expect too much in
that respect when the data have a mean I/sig()I ~ 4 .
However, looking at the chemical nature of this molecule, its sequence
is SER-TYR-SER-GLY-TYR-SER, so is it really a macromolecule? It would have
been called a hexapeptide if it had been solved by X-ray crystallography.
With best wishes,
Gerard.
--
On Tue, Jun 09, 2020 at 04:11:40PM +0200, Tobias Beck wrote:
> Dear all,
>
> Thanks a lot! (I should have used the PDB query myself for neutrons, sry,
> my bad)
>
> As there was a request to share the bioRxiv links, here they are:
> https://www.biorxiv.org/content/10.1101/2020.05.21.106740v1
> https://www.biorxiv.org/content/10.1101/2020.05.22.110189v1
>
> along with the comment in Nature (which also has both links to the papers
> in the references section):
> https://www.nature.com/articles/d41586-020-01658-1
>
> So:
> X-ray at 0.48 A
> Neutron at 0.93 A (hybrid with X-ray) or 1.05 A
> Cryo EM 1.25 A
> electron diffraction 0.6 A
>
> Thanks to all of you!
>
> Best, Tobias.
>
>
> On Tue, Jun 9, 2020 at 3:46 PM Tobias Beck <[email protected]> wrote:
>
> > Dear all,
> >
> > Thanks for the link to the latest BioRxiv papers! So for cryo EM it is 1.2
> > now. Any numbers for neutron?
> >
> > Best, Tobias.
> >
> > Tobias Beck <[email protected]> schrieb am Di. 9. Juni 2020 um 15:35:
> >
> >> Dear all,
> >>
> >> I was asked by a student what the highest resolution is, for each of the
> >> four methods listed above. Maybe someone has researched the current numbers
> >> previously and would like to share them? For X-ray, I found 0.48 A in the
> >> PDB. For EM method details, the PDB gives me 0.6 A, but it is actually for
> >> electron diffraction. I found a structure with 1.8 A for Cryo EM.
> >>
> >> I am aware that resolution is only one parameter and that high resolution
> >> may not correspond to high data quality. However, maybe someone knows the
> >> record holders, either for biomacromolecules or small molecules or for
> >> both.
> >>
> >> Thanks!
> >>
> >> Best, Tobias.
> >>
> >>
> >>
> >>
>
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