I vaguely recall that using MUSTANG you will get the distance between residues 
reflected in the b-value column and then you can color by B-value.

https://lcb.infotech.monash.edu/mustang/mustang_psfb-final.pdf 
<https://lcb.infotech.monash.edu/mustang/mustang_psfb-final.pdf>

Jürgen 

> On May 26, 2021, at 11:28 AM, Harry Powell - CCP4BB 
> <[email protected]> wrote:
> 
> Hi Jurgen
> 
> NMR structures don’t appear to have B_factors, or at least not meaningful 
> ones (e.g. in 2kv5 they’re all 0.00…). 
> 
> thanks for the response, though
> 
> Harry
> 
>> On 26 May 2021, at 16:21, Jurgen Bosch <[email protected]> wrote:
>> 
>> How about color by B-factor and look for the cold areas and hot areas?
>> Jürgen 
>> 
>>> On May 26, 2021, at 11:04 AM, Harry Powell - CCP4BB 
>>> <[email protected]> wrote:
>>> 
>>> Hi
>>> 
>>> Given that there are plenty of people on this BB who are structural 
>>> biologists rather than “just” crystallographers, I thought someone here 
>>> might be able to help.
>>> 
>>> If I have a structure in the PDB (e.g. 2kv5) that is an ensemble of 
>>> structures that fit the NOEs, is there a tool available that will give me 
>>> some idea about the bits of the structure that do not vary much (“rigid”) 
>>> and the bits that are all over the place (“flexible”)?
>>> 
>>> Would superpose or gesamt be a good tool for this? Ideally I’d like 
>>> something that could add a figure to the B columns in a PDB file so I could 
>>> see something in QTMG (or PyMol if forced…) or do other useful things with 
>>> the information.
>>> 
>>> Harry
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