It is possible that you could address some of your questions
more quickly and much more cheaply by small-angle scattering,
either light (SAS) or X-ray (SAXS).
I would suggest looking into those avenues first.
If you have well behaved (i.e. non-aggregating) purified proein
and access to synchrotron beam time (easily requested),
a series of SAXS experiments could probably be conducted in one day.
I don't want to oversell SAXS, I'm not really an enthusiast.
But this case, categorizing the interaction of two poorly ordered proteins
in solution and in particular the facilitation or disruption of this
interaction by small molecules, should be well within its scope.
best
Ethan
On Saturday, 14 August 2021 14:12:40 PDT Sorin Draga wrote:
> Hello everyone,
>
> I do realize that this is not a NMR focused group, but I do hope that there
> are a few spectroscopists lurking around that could possibly answer a few
> questions (I am more of a modeler/computationalist):
>
> The problem: I have two intrinsically disordered proteins that are known to
> interact (let's call them 1 and 2). I would like to get structural
> information (a conformational ensemble) for 1 and for the "complex" (1+2).
> Further down the line (depending on whether this is possible) I would also
> like to evaluate potential small molecule inhibitors for the said complex.
> Both 1 and 2 are <200 aminoacids long.
>
> The questions:
>
> 1. Could the cost of determining the "structure" for 1 and 1+2 be
> estimated? To be more precise, I am looking for a ball-park figure on how
> much a NMR measurement would cost in this case.
> 2. Could anyone recommend a good group/CRO that could provide such a
> service and not have an astronomical cost?
> 3. Any other suggestions/thoughts that you think might be worth mentioning
> (minimum quantity of protein necessary, purity, type of NMR etc)
>
> Many thanks for your help and time!
>
> Cheers!
>
> Sorin
>
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--
Ethan A Merritt
Biomolecular Structure Center, K-428 Health Sciences Bldg
MS 357742, University of Washington, Seattle 98195-7742
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