Hello, my numpty-level understanding is that being intrinsically disorder and giving high-resolution structural data are mutually exclusive. I will re-read your e-mails. Hope this helps. Cheers, Jon.C.
Sent from ProtonMail mobile -------- Original Message -------- On 15 Aug 2021, 09:16, Sorin Draga wrote: > Hello Ethan, > > Thank you for the suggestions. I should have mentioned in my initial post > that my intention is to first conduct a high throughput virtual screening on > these proteins, thus I would need high "resolution" of the structures which > SAXS could not provide, as far as I understand. > SAXS/SAS might become useful at a later stage, when I have a small number of > potential inhibitors identified. > Kind regards, > Sorin > > On Sat, Aug 14, 2021 at 5:55 PM Ethan A Merritt <[email protected]> wrote: > >> It is possible that you could address some of your questions >> more quickly and much more cheaply by small-angle scattering, >> either light (SAS) or X-ray (SAXS). >> >> I would suggest looking into those avenues first. >> >> If you have well behaved (i.e. non-aggregating) purified proein >> and access to synchrotron beam time (easily requested), >> a series of SAXS experiments could probably be conducted in one day. >> I don't want to oversell SAXS, I'm not really an enthusiast. >> But this case, categorizing the interaction of two poorly ordered proteins >> in solution and in particular the facilitation or disruption of this >> interaction by small molecules, should be well within its scope. >> >> best >> >> Ethan >> >> On Saturday, 14 August 2021 14:12:40 PDT Sorin Draga wrote: >>> Hello everyone, >>> >>> I do realize that this is not a NMR focused group, but I do hope that there >>> are a few spectroscopists lurking around that could possibly answer a few >>> questions (I am more of a modeler/computationalist): >>> >>> The problem: I have two intrinsically disordered proteins that are known to >>> interact (let's call them 1 and 2). I would like to get structural >>> information (a conformational ensemble) for 1 and for the "complex" (1+2). >>> Further down the line (depending on whether this is possible) I would also >>> like to evaluate potential small molecule inhibitors for the said complex. >>> Both 1 and 2 are <200 aminoacids long. >>> >>> The questions: >>> >>> 1. Could the cost of determining the "structure" for 1 and 1+2 be >>> estimated? To be more precise, I am looking for a ball-park figure on how >>> much a NMR measurement would cost in this case. >>> 2. Could anyone recommend a good group/CRO that could provide such a >>> service and not have an astronomical cost? >>> 3. Any other suggestions/thoughts that you think might be worth mentioning >>> (minimum quantity of protein necessary, purity, type of NMR etc) >>> >>> Many thanks for your help and time! >>> >>> Cheers! >>> >>> Sorin >>> >>> ######################################################################## >>> >>> To unsubscribe from the CCP4BB list, click the following link: >>> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >>> >>> This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing >>> list hosted by www.jiscmail.ac.uk, terms & conditions are available at >>> https://www.jiscmail.ac.uk/policyandsecurity/ >>> >> >> -- >> Ethan A Merritt >> Biomolecular Structure Center, K-428 Health Sciences Bldg >> MS 357742, University of Washington, Seattle 98195-7742 > > --------------------------------------------------------------- > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
