Cool, typed a long reply but only about 1/3 of it is still relevant and is given below.
The crystal would have to be capillary mounted for these data collections or the humidity controlled somehow. Only 253 K would be a good one to use. Pre-cryo, this used to give good data at SRS! Interested in the oil method you mentioned. Anyway, for capillary work: 253 K = -20 C = good temp to use 273 K = 0 C = maybe OK 293 K = 20 C = OK 313 K = 30 C = maybe OK > Re: In addition, I computed the wilson B.s 253 - 41 273 - 35.4 293 - 36.5 313 - 0.19 Assuming the last one is a typo, unless it's a neutron dataset - no B-factor ;-) Cheers, Jon.C. Sent from ProtonMail mobile -------- Original Message -------- On 8 Sep 2022, 14:39, Matt McLeod wrote: > Hi all, This is my first JISC post so I am still working on how to navigate > this. I have to say I feel like there is a much better way to have a forum on > structural biology... I could imagine a discord server where we can post > individual questions, have live chats, etc. I'd be happy to set this up if > people are interested. The data collection was done on a single crystal, > using vector scanning to minimize radiation damage, and using oil to prevent > dehydration. I have since gone back and re-processed the 313K dataset with an > updated DIALS and the problem seems to have gone away (and gave me better > scaling statistics)...my guess is that the scaling program is just improving > some of these "bugs". This high temp dataset now has the highest average > B-factor. Regardless, there does seem to be not much variation with the > temperature at the modest temperatures which may be in line with the > biological story but these values make much more sense now. Thanks everyone > for all the suggestions with regards to scaling the data together, SCALEIT, > etc. I have a few different options to interpret the data now. Matt > ######################################################################## To > unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message > was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by > www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
