Hi Eike, we bought a Biorad back in 2005 or so, an Akta Purifier in 2011 and recently an Akta Go (2020). All work(ed) well, the latter two are in use. Don't know about the Biorad, it's in Santiago de Compostela. Both the Biorad and Purifier have been used a lot, the Akta Go not that much so far. Just because the programs are set up for the Purifier I think, not because people like it more per se. For "normal" protein purifications all are fine. We've had breakdowns in both the Biorad and Purifier, which could be fixed with not too much spending. I'm not sure about this kind of equipment in a multi-user environment, best to keep it in one group I think, otherwise it's difficult people feel responsible enough to use them properly. This is even more important for columns, they are easily ruined if just once an incorrectly prepared sample is run through. Price: we got very good deals on the two Akta systems we bought, not more expensive than a comparable Biorad. But Cytiva service/repair is very expensive. Good luck, Mark
Mark J van Raaij Dpto de Estructura de Macromoleculas, lab 20B Centro Nacional de Biotecnologia - CSIC calle Darwin 3 E-28049 Madrid, Spain tel. +34 91 585 4616 (internal 432092) Section Editor Acta Crystallographica F https://journals.iucr.org/f/ https://namedrop.io/markvanraaij > On 16 Sep 2022, at 09:39, Schulz, Eike-Christian <[email protected]> > wrote: > > Dear all, > > I am considering to purchase a chromatography system for routine protein > purification. The device is supposed to be used in a multi-user environment, > hence ease of use, ease of training and ease of maintenance is important. I > am rather looking for a robust system people like to use than a system that > comes with many bells and whistles that no one dares to touch. > > Is there any particularly _bad_ experience with either system? > > Is there any specific advantage of one system over the other? > > Does anyone have experience about (long-term) stability / performance of the > systems? > > Do you think the higher price-point of the Aekta systems is justified? > > > With best regards, > > Eike > > > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
