Hi Eike, Instead of AKTA Pure I purchased a combo of AKTA Go (€24k with air sensor) and AKta start (€9k with frac collector). It provides two parallel workstations with AKTA start handling the dirty job of loading lysates onto affinity cartridges and the GO doing clean SEC stages. For multiple proteins isolation, this combo beats AKTA Pure flat out for half price. Note that AKTA Start can’t be equipped with an air sensor, and sample loading need to be done by time control. However, we didn’t find it to be an issue. Also, AKTA Start needs a separate version of Uncocrn suite for an extra €1k. So we skipped that and control the system via the built-in touchscreen.
As for the longevity, we need to see. Six months into active operation went smooth—the only malfunction was the Windows update breaking network card drivers. Best, Oleksiy Dr Oleksiy Kovtun Max-Planck Research Group Leader Molecular Mechanisms of Membrane Trafficking Max-Planck-Institute for Multidisciplinary Sciences City-Campus, Hermann-Rein-Str. 3, 37075 Göttingen Germany Email: [email protected]<mailto:[email protected]> https://www.mpinat.mpg.de/kovtun Phone:+49-551-3899-410 On 16. Sep 2022, at 09:39, Schulz, Eike-Christian <[email protected]<mailto:[email protected]>> wrote: Dear all, I am considering to purchase a chromatography system for routine protein purification. The device is supposed to be used in a multi-user environment, hence ease of use, ease of training and ease of maintenance is important. I am rather looking for a robust system people like to use than a system that comes with many bells and whistles that no one dares to touch. * Is there any particularly _bad_ experience with either system? * Is there any specific advantage of one system over the other? * Does anyone have experience about (long-term) stability / performance of the systems? * Do you think the higher price-point of the Aekta systems is justified? With best regards, Eike ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
