Hi Oleksiy,
If the Akta Start is just for loading affinity columns (without air
sensor or software), why not to get a Cyvita peristaltic pump
<https://www.cytivalifesciences.com/en/us/shop/chromatography/tools-and-accessories/pumps/peristaltic-pump-p-1-p-00564>
for £3k rather than £9k?
They are really good work horses in my experience; they are also
overpriced but still much cheaper than the rest?
BW,
D
On 16/09/2022 15:48, Kovtun, Oleksiy wrote:
Hi Eike,
Instead of AKTA Pure I purchased a combo of AKTA Go (€24k with air
sensor) and AKta start (€9k with frac collector). It provides two
parallel workstations with AKTA start handling the dirty job of
loading lysates onto affinity cartridges and the GO doing clean SEC
stages. For multiple proteins isolation, this combo beats AKTA Pure
flat out for half price. Note that AKTA Start can’t be equipped with
an air sensor, and sample loading need to be done by time control.
However, we didn’t find it to be an issue.
Also, AKTA Start needs a separate version of Uncocrn suite for an
extra €1k. So we skipped that and control the system via the built-in
touchscreen.
As for the longevity, we need to see. Six months into active operation
went smooth—the only malfunction was the Windows update breaking
network card drivers.
Best,
Oleksiy
Dr Oleksiy Kovtun
Max-Planck Research Group Leader
Molecular Mechanisms of Membrane Trafficking
Max-Planck-Institute for Multidisciplinary Sciences
City-Campus, Hermann-Rein-Str. 3, 37075 Göttingen
Germany
Email: [email protected] <mailto:[email protected]>
https://www.mpinat.mpg.de/kovtun
Phone:+49-551-3899-410
On 16. Sep 2022, at 09:39, Schulz, Eike-Christian
<[email protected]> wrote:
Dear all,
I am considering to purchase a chromatography system for routine
protein purification. The device is supposed to be used in a
multi-user environment, hence ease of use, ease of training and ease
of maintenance is important. I am rather looking for a robust system
people like to use than a system that comes with many bells and
whistles that no one dares to touch.
* Is there any particularly _/bad/_ experience with either system?
* Is there any specific advantage of one system over the other?
* Does anyone have experience about (long-term) stability /
performance of the systems?
* Do you think the higher price-point of the Aekta systems is
justified?
With best regards,
Eike
------------------------------------------------------------------------
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1>
------------------------------------------------------------------------
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1>
--
Dom Bellini, Xray Crystallography Facility (1S205)
MRC Laboratory of Molecular Biology
Francis Crick Avenue
Cambridge Biomedical Campus
Cambridge CB2 0QH
Phone 01223 267839
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list
hosted by www.jiscmail.ac.uk, terms & conditions are available at
https://www.jiscmail.ac.uk/policyandsecurity/
