A and B unit cell dimensions are hardly bigger than twice the uni cell of cubic NaCl and will probably not accommodate a 30 kDa protein.
Make a SDS gel of washed & dissolved crystals to be sure these are not alt or inhibitor crystals. You can stain the crystals with IzIt... J. -- Dr. math. et dis. nat. Jeroen R. Mesters https://orcid.org/0000-0001-8532-6699 University of Lübeck Institute of Biochemistry https://www.biochem.uni-luebeck.de phone: +49-451-3101-3105 Ratzeburger Allee 160 23562 Lübeck Germany -- > Am 03.02.2023 um 09:22 schrieb kavyashreem <[email protected]>: > > Dear all, > > We crystallized a protein (30kDa) + ligand (by cocrystallization), in the > condition 10%PEG3350, 50mM Zinc acetate. > Protein was in the buffer 20mM HEPES, 150mM NaCl, 1uM ZnCl2, 4mM TCEP, pH 8. > Crystal: Crystal: > crystal under UV m > <b06fc576.png> <e091c7fd.png> <8ef9453e.png> > When we collected the data at an in-house facility, it looked something like > this: > <b903961d.png> > The minimum resolution spot is around 9Ang and maximum ~2.2Ang. > I have not come across a protein diffraction like this, nor of a salt. When I > ran the gel for the incubated protein (protein+ligand), there was no > degradation. > Although, I was sure there is some problem with this image I tried > processing, which could not be, But indexing showed a unit cell of 11Ang, > 11Ang, 46Ang in P3. which was quite expected for two of the axes but not the > third. > Can anyone please shed some light on this diffraction image? > How can it happen? > > Thank you > Regards > Kavya > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
