like others mentioned, looks like something in between a salt and a protein, perhaps TCEP, the ligand, a peptide cleaved from your protein by trace protease. If possible, I would move the detector closer, collect an atomic resolution dataset and try to solve the structure by direct methods. You never know, it could be something interesting.
Mark J van Raaij Dpto de Estructura de Macromoleculas, lab 20B Centro Nacional de Biotecnologia - CSIC calle Darwin 3 E-28049 Madrid, Spain tel. +34 91 585 4616 (internal 432092) Section Editor Acta Crystallographica F https://journals.iucr.org/f/ https://namedrop.io/markvanraaij > On 3 Feb 2023, at 09:22, kavyashreem <kavyashr...@instem.res.in> wrote: > > Dear all, > > We crystallized a protein (30kDa) + ligand (by cocrystallization), in the > condition 10%PEG3350, 50mM Zinc acetate. > Protein was in the buffer 20mM HEPES, 150mM NaCl, 1uM ZnCl2, 4mM TCEP, pH 8. > Crystal: Crystal: > crystal under UV m > <b06fc576.png> <e091c7fd.png> <8ef9453e.png> > When we collected the data at an in-house facility, it looked something like > this: > <b903961d.png> > The minimum resolution spot is around 9Ang and maximum ~2.2Ang. > I have not come across a protein diffraction like this, nor of a salt. When I > ran the gel for the incubated protein (protein+ligand), there was no > degradation. > Although, I was sure there is some problem with this image I tried > processing, which could not be, But indexing showed a unit cell of 11Ang, > 11Ang, 46Ang in P3. which was quite expected for two of the axes but not the > third. > Can anyone please shed some light on this diffraction image? > How can it happen? > > Thank you > Regards > Kavya > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
