I guess you must have CCP4 software installed?
Try setting up a project with CCP4I2 and running the task under
Xray data reduction and analysis
  Data reduction - AIMLESS

This will give you a detailed report on possible spacegroups and indexing..
Very helpful for a beginner!


On Fri, 17 Jul 2026 at 08:03, Kay Diederichs <
[email protected]> wrote:

> Hello Jakob,
>
> The highest-possible crystal symmetry should always be used, in order to
> make the description of the experiment as simple (parsimonious) as possible.
>
> In my book, the R-values you report are the "same" for P21 and P212121
> ("same" meaning "similar enough to be explained by different choice of free
> reflections, and different degrees of freedom resulting from having twice
> as many atoms"). That means that the higher symmetry is the correct one.
>
> Just note that you can _always_ refine in a lower-symmetry subgroup,
> without really changing the scientific insight from the experiment.
> P21 is a subgroup of P212121. Note that in your case, you can choose the
> unique axis of P21 in three ways - you chose one of them. The other two
> should give similar R-values.
> You could even refine in P1 (of course with 4 times the number of
> molecules in the ASU, compared to P212121, and twice compared to P21),
> without significantly changing the R-values.
>
> Hope this helps,
> Kay
>
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