Hello,

I wonder if this feature was changed in the recent versions of Coot? Is it
possible to refine phosphate, sugar and base separately?

I am using Coot and wincoot 0.33 and apparently none of them allows this.

Thanks in advance for your replies.

Ricardo


From: Paul Emsley <>
Date: Tue Nov 21 2006 - 17:58:19 GMT

On Tue, Nov 21, 2006 at 06:07:48PM +0100, Kristina Lakomek wrote:
>
> I would like to build a ds DNA in Coot. Does anyone know, whether it is
> possible to modify / refine the base, the ribose and the phosphate part of
> each nucleotide separately?

No, you can't refine the parts separately. I am not convinced that
that is a good idea.

You can of course do mutations and move around the individual atoms
as you can with any other residue type.

You can trivially change from C3'-endo to C2'-endo puckering by pulling
on the appropriate atom during regularization (or refinement).

Paul.

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