Hello Paul, Yes, I did some model building using Coot only of chain A. I checked the link, it is saying: Note that you can currently only apply NCS edits to residues that exist in the "other" chains. If for example, you had introduced/added residues 1 to 5 in the "A" chain and wanted to copies those over to the other molecules, then that would not work (88)<http://www.ysbl.york.ac.uk/%7Eemsley/coot/doc/chapters/user-manual_fot.html#FOOT88> .
Since I did renumbering, adding ,deleting residues to chain A, so seems it wouldnot work by apply NCS. Now I would like to run refmac5, so I need a complete model, right? I am a rather beginner, is there any better ways to rebuild in my case(4 molecules in one assymetry unit). thanks On Sun, Aug 10, 2008 at 9:36 PM, Paul Emsley <[EMAIL PROTECTED]>wrote: > jenny flower wrote: > >> Hello, >> >> I have 4 chains in my pdb, I SSM chain B,C,D to chain A. If I refined >> chain A, can regenerate a pdb with whole 4 chains from refined chain A, by >> applying SSM matrix? I see Xterm window show SSM matrix when I do SSM, but >> somewhere a log file contain this matrix information also? >> > > Dear Qing/Jenny Flower, > > When you say "I refined chain A", I am presuming that you mean that you > have done some model building using Coot. You then want to apply the edits > that you have made each of the chains in your model (applying the NCS > transformations as necessary): > > You should read the user manual, section 5.27 Applying NCS Edits: > > > http://www.ysbl.york.ac.uk/~emsley/coot/doc/chapters/user-manual_5.html#SEC134<http://www.ysbl.york.ac.uk/%7Eemsley/coot/doc/chapters/user-manual_5.html#SEC134> > > In your case it seems that you want this function: > > (copy-from-ncs-master-to-others imol master-chain-id) > > HTH, > > Paul. > >
