Dear Taufik 2008/11/17 Taufik Al-Sarraj <[EMAIL PROTECTED]>: > Hello everyone, > > I am new to Coot, I am trying to do a DNA mutation but unfortunately i have > not been successful. I am using Mutate Residue Range. > The error message i get is > > 'INFO:: mutate 2 to a ALA > DISASTER! Not all necessary atoms found in residue 2 > CA is missing > C is missing > N is missing > failure to get orientation matrix' >
If I understand correctly you try to rebuild a stretch of DNA. Clearly the error message indicates that you try to mutate the DNA to protein. Or am I totally mistaken. Anyway, this does not make any sense to me. > I tried the do mutation with a pdb structure from pdb.org <http://pdb.org> > 1DGC, and i tried to do a mutation on a dna created using nucgen in amber. > Both attempts gave me the error above. > Try reading the manual. As far as I can see you can use "Simple mutate" to mutata DNA sequences when they are named appropriately. >From section 5.14 in the manual > any help would be greatly appreciated. > Taufik > Hope this helps. Folmer
