I see. But how to deal with the situation when I hit the C/N terminal atom of the chain when using Baton mode? Like in my case, I started with an atom and after I mapped 38th of the Ca atoms, I hit the first atom (N terminus). But the Baton trades can only extend following the sequence of the number and I want to trace from where I started and went to the other direction? Do you have any good suggestion? I am very new to this, please bare with me if I asked stupid questions. Best, Yangqi
Sent from Mail for Windows 10 From: Paul Emsley Sent: Tuesday, October 2, 2018 1:44 PM To: BuddySphinx; [email protected] Subject: Re: Building backwards in Baton mode On 02/10/2018 18:22, BuddySphinx wrote: > > Thank you for your reply. However, I encountered another issue. I was able to > reverse my fragments and now > the first atom is at the correct position. But how do I continue to Baton > mode? Because when I hit Baton > mode, it seems started a new project and I have to restart the model > building? How do I continue from my > unfinished Ca tracing? Sadly the whole mechanism of dealing with fragments is not convenient. There is no "continue" baton atom molecules after you have built them. What I do is do the Ca Zone -> Mainchain and deal with mainchain fragments rather than CA fragments. With mainchain fragments, I can change the residue numbers as needed (to match other mainchain fragments) and then I would do a "merge molecules" to bring the main-chain fragments into the same molecule - and then change the chain ids to bring them into the same chain (if that's what I wanted to do - it often is (when I do this sort of thing)). Merging molecules and annealing chains is something I would do after having done several (or as many as I can) Baton Builds and subsequence CA Zone -> mainchains. Regards, Paul. ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=COOT&A=1
