Fantastic, thanks for the quick response Paul.

Best wishes,
Karim

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________________________________
From: Paul Emsley <[email protected]>
Sent: Wednesday, November 20, 2019 6:35:50 PM
To: Karim Rafie <[email protected]>; [email protected] <[email protected]>
Subject: Re: Map smoothening in Coot



On 20/11/2019 13:28, Karim Rafie wrote:



I have a question regarding the map smoothening option in coot (Calculate -> 
Map Tools -> Make a (very) smooth copy). If I understand correctly, the Shannon 
sampling factor applied by default is 1.5 and that the smoothening process 
adjusts that factor.

Does that have any effect on the validity of the map, i.e. can I still use it 
for model builduing or will it "smoothen-out" possibly important data?



"Smoother" in this case means "more finely sampled" - it should have little 
effect on the map (perhaps 1 part in 1000 or so).

It doesn't mean "blur."


0.9-pre users can use Cryo-EM -> Sharpen/Blur with a B-factor of 0 and a 
re-sample factor of 1.5 or so. That should be faster.


Paul.


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