On 16/11/2020 16:26, Moustafa, Ibrahim M. wrote:
Hi all,
I'd like to build a C-alpha model to fit a low-res cryoEM map based on a
homologous C-alpha model template.
I tried to use coot "use clustal for alignment then mutate", the alignment is generated but when I try to
mutate listed residues of 'residue mismatches' I get an error message: "Failure to get orientation matrix".
I guess it needs all-atom model to work!
At least a mainchain model, I think.
I wonder if there is a way around in coot (or other tools) to create this low resolution model using
C-alpha template.
Calculate -> Other Modelling Tools -> CA Zone to Mainchain
Then Use ClustalW for alignment on the "Forwards" molecule.
Paul.
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