On 09/12/2020 12:44, manikandank wrote:



I would like to build a model of both single and double strand RNA into a 3A cryoEM map.

In this regard, i would like to know the options in coot to do the following,

How to do real space refinement of ds RNA (different kind of RNA secondary structures) , both strand together to the fit the map (bases come from different regions of RNA primary sequence) ?


Have you watched the "Coot with RNA" video?

https://www.youtube.com/watch?v=Zzim3SXnDSk

See the comments.


Paul.

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