On 2020-12-09 18:26, Paul Emsley wrote: > On 09/12/2020 12:44, manikandank wrote: > >> I would like to build a model of both single and double strand RNA into a 3A >> cryoEM map. >> >> In this regard, i would like to know the options in coot to do the following, >> >> How to do real space refinement of ds RNA (different kind of RNA secondary >> structures) , both strand together to the fit the map (bases come from >> different regions of RNA primary sequence) ? > Have you watched the "Coot with RNA" video? > > https://www.youtube.com/watch?v=Zzim3SXnDSk > > See the comments. > > Paul.
Hi Paul, Thank you very much for the quick response. I have now seen the video and the comments. As i have very long RNA (~2000 bases from ribosomal rna) that makes different secondary structures, i donot have often the ideal situation show in the video. First, i do not have the ProSMART menu for 'Generate Restraint' and i use wincoot 0.9.2 EL (please see the attachment). How to generate the restraint for a selected set of bases as they come from different chain? for ex. strand1: 10 to 25 from chain A and strand2: 40-55 chain B . I would like to restraint part of a ds strand or stem loop and fit only the selection using real space refine option. thank you very much for your suggestions with kind regards Mani. ######################################################################## To unsubscribe from the COOT list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=COOT&A=1 This message was issued to members of www.jiscmail.ac.uk/COOT, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
