Hello,

If you question is about using a subset of the data for assembling the Assemblathon fish genome, then my opinion is that using only the 3 libraries that you listed will not provide enough
k-mer coverage for a de novo assembly.


                       Sébastien


nikos ioannidis a écrit :
I see that you use all the libraries but if i insert in example for the fish:

1 library of fragments625E1AAXX.1.1.fastq 625E1AAXX.1.2.fastq

1 jumping library  803DNABXX.1.1.fastq 803DNABXX.1.2.fastq

and 1 fosmid jumping library 62F6HAAXX.1.1.fastq 62F6HAAXX.1.2.fastq

Am I assembling the fish genome or this is wrong?

Thank you.



On Tue, May 29, 2012 at 10:17 PM, nikos ioannidis <niioa...@gmail.com <mailto:niioa...@gmail.com>> wrote:

    Hello,


    a question i have to make is from the assemblathon data, for
    example the parrot,
    what would be the minimum input of libraries in order to have a
    good assembly product,
    and what would be the maximum?
    if you could you give me an example or two i would appreciate it?


    Thank you,
and sorry if the question is excessive.



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