nikos ioannidis a écrit : > Hello, i noticed that sometimes the > N50 value of contigs >=100 and contigs >=500 is the same > this happens a lot when i have a good N50 value for a given genome > Is it random or is happening when the N50 value is in satisfactory levels? >
If all contigs have a length greater or equal to 500, then the metrics will be the same. It is deterministic and not random. > > Also when a N50 value is considered good enough? I don't think there is a universal value for that. > Should be above a threshold? No. > Should I use a K-mer between some boundaries? I always use 31 for Illumina HiSeq data. > (currently i run all my tests with K-mer 31, should i lower it?) It depends what type of data you have. ------------------------------------------------------------------------------ Live Security Virtual Conference Exclusive live event will cover all the ways today's security and threat landscape has changed and how IT managers can respond. Discussions will include endpoint security, mobile security and the latest in malware threats. http://www.accelacomm.com/jaw/sfrnl04242012/114/50122263/ _______________________________________________ Denovoassembler-users mailing list Denovoassembler-users@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/denovoassembler-users
