On 24 février 2014 04:22, Raja durai [bioc...@gmail.com] wrote: > À : denovoassembler-users@lists.sourceforge.net > Objet : [Denovoassembler-users] What is the minimum read length for Running > Ray-meta > > Hi Sebastien, > > We had used reads from Illumina platform for Ray-meta assembly. Each read > length was 251 bp. After adapter trimming, we used the trimmed > reads as query. Now my question is What is the minimum read length should be > considered for Ray-meta assembly ( We have read lengths ranges > from 1 to 250 bp after trimming).
Are you using paired reads ? Obviously, you want a read length of at least k nucleotides. > > And one more query, we used contig length >150 bp for further gene prediction > and blastx annotation. Is this right length for annotation? I don't know, this is not my field of expertise, really. > > Looking forward to hear from you > > Regards > Raja > > > ------------------------------------------------------------------------------ Flow-based real-time traffic analytics software. Cisco certified tool. Monitor traffic, SLAs, QoS, Medianet, WAAS etc. with NetFlow Analyzer Customize your own dashboards, set traffic alerts and generate reports. Network behavioral analysis & security monitoring. All-in-one tool. http://pubads.g.doubleclick.net/gampad/clk?id=126839071&iu=/4140/ostg.clktrk _______________________________________________ Denovoassembler-users mailing list Denovoassembler-users@lists.sourceforge.net https://lists.sourceforge.net/lists/listinfo/denovoassembler-users
