"David Heiser" <[EMAIL PROTECTED]> wrote in message news:<[EMAIL PROTECTED]>...
> Findamental is how well is the medium mixed. 10 bacteria makes sense if the
> medium is truely homogeneous in all other constituents. The problem then is
> the classic "balls-in-an-urn", since a bacterium is a finite particle, and
> everything else is not a particle. If the medium is a gel, then the issue is
> primarily one of homogenaity, and you have to take many more samples. One
> then gets involved in a designed experiment involing three dimensional
> sample locations.
> 
> David Heiser

Thank you David. It's liquid, not gel. 
I am trying to readress the question as follows:

The question is now "how to distinquish a bag that is 1000 bacterial
per ml from a bag that is 10 bacteria per ml? How many samples of 1 ml
should be taken out of a 60 ml bag? (0.9 power and 0.05 level of
significance)
Any way to estimate the variance?

Aron
.
.
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